Swedish Hasbeens Hippie Boot 6mDndbNlA

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Swedish Hasbeens Hippie Boot 6mDndbNlA
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Additionally, while it takes a bit more work to soften its light, a simple, open-faced lamp is a very flexible tool, and can easily be used for dramatic, stage-like lighting when needed. In a time when soft light is too quickly taken as a given, rather than a creative choice, it is worthwhile to surround oneself with flexible tools that resist specialization and repetition.

2. Diffusion Material

Softening light is a subtle art. Whether you are using a softbox or not, at least one roll of good diffusion material will allow you to further adjust the qualities of your key light. The angle, distance, and layering of this diffusion material will all produce subtle changes in the quality of light. A large, full roll of diffusion will equip you for interviews and many other lighting scenarios. Numerous qualities and weights of diffusion are available, but RoscoE-Colour #401 Light Rolux is a versatile starting point as it is gentle and relatively soft, but can be layered to produce a more aggressive softening effect.

3. Versatile Reflector

Next, a good white reflection surface will help fill in the shadows cast by the key light and create a pleasing smoothness. In a pinch, foam core from a craft store will do quite well, but the compact, portable, and durable Westcott 5-in-1 Reflector Disc is a more convenient and durable solution.

Reflectors are no more complex than neutral, white boards of one kind or another. You may prefer a home-brewed solution, or if easy C-Stand mounting is more important than a quickly collapsible product, perhaps you prefer a silk on a solid steel frame. Whatever your style, a good reflector should never be absent on almost any video shoot.

A quick note about foam core: while this is generally not a problem, if you are experiencing an acoustic reflection that you can’t track down, a closely-placed piece of foam core may be the culprit. To demonstrate this, talk directly into a piece of foam core and listen to the change in your voice. This effect will be far less pronounced with a thin fabric reflector.

4. Boom Pole Holder

The K-Tek Airo ABH1 Boompole Holder is an excellent, simply designed tool for placing your boom pole right where you want it using a C-Stand. These holders are simple to use, but it helps to know the following technique: because it’s difficult to adjust the angle of the boom pole once it is set up, start by setting the C-Stand at a nice height. Next, position your boom mic so that it is about three feet too low. Then use only the C-Stand riser to lift the mic into position. I like to place the C-Stand high enough to allow cast and crew to pass freely beneath the boom pole.

5. Sound Blanket

Audio is the life-blood of an interview, and dampening the worst acoustic reflections will generally do far more for your sound quality than a new high-end microphone. A Matthews Sound Blanket will come in handy here, as well as help you pack your gear up safely.

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FIG 10

Effects of SLFN5 knockdown on collagen contraction. (A) Control shRNA-GFP (upper panels) and SLFN5 shRNA-GFP (lower panels) cells (1 × 10) were plated in a 3D collagen contraction assay. The GM6001 (GM) inhibitor was added to the medium at a 10 μM final concentration, as indicated (right panels). Collagen contraction was assessed after 4 days of culture. The figure shows three independent experiments (I, II, and III). (B) The surface area of the inner gel plugs from panel A was quantified using ImageJ. The relative changes in the surface area were recorded as the percentage of the original surface area. Shown here is the percentage of collagen contraction ± standard errors of results from three independent experiments. (C) 786-0 control shRNA-GFP cells were transiently transfected with control siRNA, MMP-1 siRNA, or MMP-13 siRNA. At 48 h after transfection, gene expression of MMP-1 and MMP-13 was analyzed by real-time RT-PCR using specific primers and GAPDH as an internal control. Data are expressed as fold changes over the control and represent the mean ± standard error of results from three experiments. (D) Control shRNA-GFP (upper panels) and SLFN5 shRNA-GFP (lower panels) cells were transiently transfected with control siRNA, MMP-1 siRNA, or MMP-13 siRNA. At 48 h after transfection, the cells were plated in a 3D collagen contraction assay. Collagen contraction was assessed after 4 days of culture. The figure is representative of three independent experiments. (E) The surface area of the inner gel plugs from panel D was quantified using ImageJ. The relative changes in the surface area were recorded as the percentage of the original surface area. Shown here is the percentage of collagen contraction ± the standard error of results from three independent experiments.

Since MMPs also mediate cell migration and invasion, we next examined the effects of SLFN5 knockdown on cell motility by live-cell imaging. Cells transfected with SLFN5 siRNA demonstrated increased cell motility ( Jambu Aruba Black Sandals HOJt3k
; see also Movies S1 and S2 in the supplemental material). SLFN5 knockdown also increased migration in a transwell migration assay ( Fig. 11B and C ). In addition, SLFN5 knockdown increased invasion of 786-0 cells through Matrigel-coated transwell membranes ( Steven ValentP 18f3qyLJeu
and E ). Taken together, these results provide strong evidence for a key negative regulatory role for SLFN5 in RCC tumor progression.

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FIG 11

SLFN5 inhibits malignant cell migration. (A) 786-0 cells were transiently transfected with control siRNA or SLFN5 siRNA, as indicated; at 48 h after transfection, cells were transferred to a Nikon Biostation incubator wide-field microscope and time-lapse images were acquired at 10-min intervals for 12 h. Data were analyzed using the Nikon Elements software. Data represent the means ± standard errors of results from 27 migratory events detected by the Nikon Elements software. (B) 786-0 cells were transiently transfected with control siRNA or SLFN5 siRNA, as indicated. At 48 h after transfection cells, were plated in BD BioCoat cell culture inserts for 3 h and then cells were stained. Representative images of migrating cells (×5) after 3 h of incubation are shown. (C) The migrated cells were counted, and relative migration is expressed as the fold increase over control ± standard error of results from three independent experiments. Paired two-tailed test analysis showed a value of 0.0198 (*). (D) 786-0 cells were transiently transfected with control siRNA or SLFN5 siRNA. At 48 h after transfection, the cells were plated in a Corning BioCoat Matrigel invasion chamber, and invasion of the cells was assessed in 6 h. Representative images of invading cells (×5) after 6 h of incubation are shown. (E) The invading cells were counted, and relative invasion is expressed as the fold increase over controls ± standard errors of results from four independent experiments. Paired two-tailed test analysis showed a value of 0.02933 (*).

Since our results using renal cell carcinoma cell lines provided evidence for a potential important functional role for SLFN5 in RCC progression, we next sought to determine the expression of SLFN5 in RCC samples and whether its expression was linked to overall survival. We analyzed a recently obtained RNA-seq data set of 470 RCC samples and compared the data to 68 nontumor control kidney samples ( 28 , adidas by Stella McCartney Energy Boost KrCGk
). Although SLFN5 was significantly overexpressed in RCC samples ( t test, P value = 2 × 10 −6 ) ( Fig. 12A ), higher expression of SLFN5 was significantly associated with a better overall survival in RCC samples (log rank, P value = 0.003) ( Tory Sport Ruffle Sneaker p9Hbtx
). In addition, when the prognostic effects of MMP-1 or MMP-13 in RCC were evaluated, we found that high MMP-13 expression correlated significantly with adverse overall survival ( Miz Mooz Demi XHafc8t
), while MMP-1 independently did not show any significant correlation with survival ( Fig. 12D ).

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